Article
Rapid screening of Salmonella enterica serovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting the O and H antigen alleles.
BMC microbiology - 9 Oct 2008
Hong Yang, Liu Tongrui, Lee Margie D, Hofacre Charles L, Maier Marie, White David G, Ayers Sherry, Wang Lihua, Berghaus Roy, Maurer John J
Abstract excerpt
BACKGROUND: Classical Salmonella serotyping is an expensive and time consuming process that requires implementing a battery of O and H antisera to detect 2,541 different Salmonella enterica serovars. For these reasons, we developed a rapid multiplex polymerase chain reaction (PCR)-based typing scheme to screen for the prevalent S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium. RESULTS: By...
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