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pH-FISH: coupled microscale analysis of microbial identity and acid–base metabolism in complex biofilm samples

20 Dec 2024

Abstract excerpt

BACKGROUND: Correlative structural and chemical imaging of biofilms allows for the combined analysis of microbial identity and metabolism at the microscale. Here, we developed pH-FISH, a method that combines pH ratiometry with fluorescence in situ hybridization (FISH) in structurally intact biofilms for the coupled investigation of microbial acid metabolism and biofilm composition. Careful biofilm handling and...

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PMID: 39707459
DOI: 10.1186/s40168-024-01977-9

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