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Esterase-2 mutant-based nanostructured amperometric biosensors for the selective determination of paraoxon (Neurotoxin).

Analytical biochemistry - 1 Mar 2025

Magar Hend Samy, Fayez Muhammad, Febbraio Ferdinando, Hassan Rabeay Y A

Abstract excerpt

Organophosphate pesticides (OPs) are causing non-selective inhibition in enzymatic bioreceptors, thus the enzymatic-inhibition-based traditional assays are not suitable for their specific detection in food and environmental samples. Accordingly, a selective nanostructured electrochemical biosensing system was designed using six mutants of the esterase-2 (EST2 protein) enzymes from A. acidocaldarius to be...

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PMID: 39681172
DOI: 10.1016/j.ab.2024.115751

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