Article
Insecticide detection through protein engineering of Nippostrongylus brasiliensis acetylcholinesterase B.
Analytical chemistry - 15 Sept 2005
Schulze Holger, Muench Susanne B, Villatte Francois, Schmid Rolf D, Bachmann Till T
Abstract excerpt
The sensitivity of acetylcholinesterase (AChE) biosensors for insecticide detection could be increased substantially by engineering AChE B of Nippostrongylus brasiliensis. The introduction of 10 single and 4 double mutations into the AChE peptide chain led to an increase in sensitivity to 10 of the 11 insecticides tested. The combination of three mutants with the wild-type enzyme in a multienzyme biosensor array...
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