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Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis

13 Dec 2024

Abstract excerpt

ABSTRACT We describe a new Chlamydia trachomatis protein depletion method that uses an engineered small RNA (sRNA) to inhibit translation of a target gene. In proof-of-principle experiments, we induced functional knockdown of IncA, a fusion-mediating inclusion membrane protein, as shown with Western blots, loss of IncA staining at the inclusion membrane, and production of multiple chlamydial inclusions within an...

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PMID: 39670716
DOI: 10.1128/mbio.02545-24

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