Article
A simple, cost-effective, and efficient method for screening CRISPR/Cas9 mutants in plants.
Journal of plant physiology - 1 Dec 2024
Wang Yiping, Ma Jun, Wu Yingying, Yang Shuying, Wang Pengxi, Zhang Hailei, Li Jitong, Chen Lin, Kong Weiwen, Xia Yiji, Wang Qiong, Liu Jinglan
Abstract excerpt
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing system is widely used for targeted mutagenesis in a growing number of plant species. To streamline the screening process for mutants, especially those generated from low-efficiency editing events, there is a need for a rapid, cost-effective, and efficient method. Although several...
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