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Article

Design of a Robust Flow Cytometric Approach for Phenotypical and Functional Analysis of Human Monocyte Subsets in Health and Disease.

Biomolecules - 3 Oct 2024

Ahrazoglu Talia, Kluczny Jennifer Isabel, Kleimann Patricia, Irschfeld Lisa-Marie, Nienhaus Fabian Theodor, Bönner Florian, Gerdes Norbert, Temme Sebastian

Abstract excerpt

Human monocytes can be subdivided into phenotypically and functionally different classical, intermediate and non-classical monocytes according to the cell surface expression of CD14 and CD16. A precise identification and characterisation of monocyte subsets is necessary to unravel their role in inflammatory diseases. Here, we compared three different flow cytometric strategies (A-C) and found that strategy C,...

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Source

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Identifiers

PMID: 39456184
DOI: 10.3390/biom14101251
PMCID: PMC11506830

Genes

CD14
CD74

Provenance

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