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Evaluating the Efficacy of Target Capture Sequencing for Genotyping in Cattle.

Genes - 18 Sept 2024

Ren Yan, Khatkar Mehar S, MacPhillamy Callum, Wang Haofei, McEwin Rudi A, Chen Tong, Pitchford Wayne S, Low Wai Yee

Abstract excerpt

(1) Background: Target capture sequencing (TCS) is potentially a cost-effective way to detect single-nucleotide polymorphisms (SNPs) and an alternative to SNP array-based genotyping. (2) Methods: We evaluated the effectiveness and reliability of TCS in cattle breeding scenarios using 48 female and 8 male samples. DNA was extracted from blood samples, targeted for 71,746 SNPs with TWIST probes, and sequenced on an...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 39336809
DOI: 10.3390/genes15091218
PMCID: PMC11431841

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