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Article

Immune phenotyping in a pediatric multicenter transplant study: Suitability of a preformulated dry-antibody panel system.

Human immunology - 1 Sept 2024

Ionescu Lavinia I, Blydt-Hansen Tom, Foster Bethany J, Allen Upton, Birk Patricia E, Hamiwka Lorraine, Phan Veronique, Min Sandar, Ivison Sabine, Levings Megan, West Lori J, Mital Seema, Urschel Simon

Abstract excerpt

Flow-cytometric immune phenotyping is influenced by cryopreservation and inter-laboratory variability limiting comparability in multicenter studies. We assessed a system of optimized, pre-mixed dry-antibody panel tubes requiring small amounts of whole blood for validity, reliability and challenges in a Canadian multicenter study (POSITIVE) with long-distance sample shipping, using standardized protocols....

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PMID: 39013208
DOI: 10.1016/j.humimm.2024.110837

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