Age Distribution of Multiple Functionally Relevant Subsets of CD4+ T Cells in Human Blood Using a Standardized and Validated 14-Color EuroFlow Immune Monitoring Tube.

doi:10.3389/fimmu.2020.00166

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Article

Age Distribution of Multiple Functionally Relevant Subsets of CD4+ T Cells in Human Blood Using a Standardized and Validated 14-Color EuroFlow Immune Monitoring Tube.

Frontiers in immunology - 1 Jan 2020

Botafogo Vitor, Pérez-Andres Martín, Jara-Acevedo María, Bárcena Paloma, Grigore Georgiana, Hernández-Delgado Alejandro, Damasceno Daniela, Comans Suzanne, Blanco Elena, Romero Alfonso, Arriba-Méndez Sonia, Gastaca-Abasolo Irene, Pedreira Carlos Eduardo, van Gaans-van den Brink Jacqueline A M, Corbiere Véronique, Mascart Françoise, van Els Cécile A C M, Barkoff Alex-Mikael, Mayado Andrea, van Dongen Jacques J M, Almeida Julia, Orfao Alberto

Abstract excerpt

CD4+ T cells comprise multiple functionally distinct cell populations that play a key role in immunity. Despite blood monitoring of CD4+ T-cell subsets is of potential clinical utility, no standardized and validated approaches have been proposed so far. The aim of this study was to design and validate a single 14-color antibody combination for sensitive and reproducible flow cytometry monitoring of CD4+ T-cell...

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PMID: 32174910
DOI: 10.3389/fimmu.2020.00166
PMCID: PMC7056740

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