Article
A General Protocol for Gene Expression Analysis in Chemically Mutant Coffee Plants in Response to Rust (Hemileia vastatrix Berk & Broome) Infection.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2024
Gatica-Arias Andrés, Muñoz-Flores Carolina, Bolívar-González Alejandro
Abstract excerpt
Coffea spp. is the source of one of the most widely consumed beverages in the world. However, the cultivation of this crop is threatened by Hemileia vastatrix Berk & Broome, a fungal disease, which reduces the productivity and can cause significant economic losses. In this protocol, coffee leaf segment derived from a chemical mutagenesis process are inoculated with uredospores of the pathogen. Subsequently, the...
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