Article
A bioorthogonal cell sorting strategy for isolation of desired cell phenotypes.
Chemical communications (Cambridge, England) - 13 Feb 2024
Dai Liqun, Zhou Siming, Yang Cheng, Li Jie, Wang Yayue, Qin Meng, Pan Lili, Zhang Dan, Qian Zhiyong, Wu Haoxing
Abstract excerpt
Here we describe a cost-effective and simplified cell sorting method using tetrazine bioorthogonal chemistry. We successfully isolated SKOV3 cells from complex mixtures, demonstrating efficacy in separating mouse lymphocytes expressing interferon and HeLa cells expressing virally transduced green fluorescent protein post-infection.
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