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Random mutagenesis of Phaeodactylum tricornutum using ultraviolet, chemical, and X-radiation demonstrates the need for temporal analysis of phenotype stability.

Scientific reports - 16 Dec 2023

Macdonald Miller Sean, Abbriano Raffaela M, Herdean Andrei, Banati Richard, Ralph Peter J, Pernice Mathieu

Abstract excerpt

We investigated two non-ionising mutagens in the form of ultraviolet radiation (UV) and ethyl methanosulfonate (EMS) and an ionising mutagen (X-ray) as methods to increase fucoxanthin content in the model diatom Phaeodactylum tricornutum. We implemented an ultra-high throughput method using fluorescence-activated cell sorting (FACS) and live culture spectral deconvolution for isolation and screening of potential...

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Identifiers

PMID: 38104215
DOI: 10.1038/s41598-023-45899-2
PMCID: PMC10725415

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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