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Is qPCR always the most sensitive method for malaria diagnostic quality surveillance?

15 Dec 2023

Abstract excerpt

In many studies to evaluate the quality of malaria diagnosis, microscopy or rapid diagnostic tests (RDT) are compared to PCR. Depending on the method for sample collection and storage (whole blood or dried blood spot), volume of blood used for extraction, volume of DNA used as PCR template, and choice of PCR target (single vs. multi-copy gene), the limit of detection (LOD) of PCR might not exceed the LOD of...

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PMID: 38102649
DOI: 10.1186/s12936-023-04822-w

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