Article
Synchronous detection of Burkholderia pseudomallei and its ceftazidime resistance mutation based on RNase-HII hydrolysis combined with lateral flow strip assay.
Microbiology spectrum - 12 Dec 2023
Yao Juan, Zhang Zhang, Tian Shen, Luo Nini, Tan Jun, Zhang Yue, Gu Shuo, Xia Qianfeng
Abstract excerpt
IMPORTANCE: This study focused on the development of a reaction system using rhPCR to amplify a specific gene, ORF2, of B. pseudomallei and to identify the P174L mutation associated with increased drug resistance to ceftazidime (CAZ). The system incorporated universal primer probes and a simple temperature cycle reaction. The amplified products were then analyzed using lateral flow strip assay (LFSA) for strain...
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