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Highly specific and sensitive detection of Burkholderia pseudomallei genomic DNA by CRISPR-Cas12a

29 Aug 2022

Abstract excerpt

Detection of Burkholderia pseudomallei, a causative bacterium for melioidosis, remains a challenging undertaking due to long assay time, laboratory requirements, and the lack of specificity and sensitivity of many current assays. In this study, we are presenting a novel method that circumvents those issues by utilizing CRISPR-Cas12a coupled with isothermal amplification to identify B. pseudomallei DNA from...

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PMID: 36037185
DOI: 10.1371/journal.pntd.0010659

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