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Article

Protocol for isolating human BCAM-positive corneal progenitor cells by flow cytometry and cell sorting

1 Sept 2023

Abstract excerpt

BCAM-positive basal limbal epithelial cells are an early transit-amplifying cell population (TAC) capable of holoclone formation and corneal epithelial differentiation. Here, we present a protocol for isolating BCAM-positive cells from human donor corneas by flow cytometry and cell sorting. We describe steps for cell dissection and dissociation, antibody staining, and flow cytometry. We then detail procedures for...

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Identifiers

PMID: 37669162
DOI: 10.1016/j.xpro.2023.102503

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