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Safe-Harbor-Targeted CRISPR/Cas9 System and Cmhyd1 Overexpression Enhances Disease Resistance in Cordyceps militaris

9 Oct 2023

Abstract excerpt

Fungal disease of mushroom Cordyceps militaris (CM) caused by Calcarisporium cordycipiticola (CC) is destructive to fruiting body cultivation, resulting in significant economic loss and potential food safety risks. CRISPR/Cas9 genome editing has proven to be a powerful tool for crop improvement but seldom succeeded in mushrooms. Here, the first genomic safe-harbor site, CmSH1 locus, was identified in the CM...

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PMID: 37807760
DOI: 10.1021/acs.jafc.3c05131

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