Article
Site-directed mutagenesis of Mycobacterium tuberculosis and functional validation to investigate potential bedaquiline resistance-causing mutations.
Scientific reports - 6 Jun 2023
Otum Christian C, Rivière Emmanuel, Barnard Monique, Loubser Johannes, Williams Monique J, Streicher Elizabeth M, Van Rie Annelies, Warren Robin M, Klopper Marisa
Abstract excerpt
Molecular detection of bedaquiline resistant tuberculosis is challenging as only a small proportion of mutations in candidate bedaquiline resistance genes have been statistically associated with phenotypic resistance. We introduced two mutations, atpE Ile66Val and Rv0678 Thr33Ala, in the Mycobacterium tuberculosis H37Rv reference strain using homologous recombineering or recombination to investigate the...
Topics
- Humans
- Mycobacterium tuberculosis
- Antitubercular Agents
- Diarylquinolines
- Mutation
- Microbial Sensitivity Tests
- Mutagenesis, Site-Directed
- Tuberculosis, Multidrug-Resistant