Article
Mutational analysis of the functional motifs of the DEAD-box RNA helicase Me31B/DDX6 in Drosophila germline development.
FEBS letters - 1 Jul 2023
Kara Evan, McCambridge Aidan, Proffer Megan, Dilts Carol, Pumnea Brooke, Eshak John, Smith Korey A, Fielder Isaac, Doyle Dominique A, Ortega Bianca M, Mukatash Yousif, Malik Noor, Mohammed Ammaar R, Govani Deep, Niepielko Matthew G, Gao Ming
Abstract excerpt
Me31B/DDX6 is a DEAD-box family RNA helicase playing roles in post-transcriptional RNA regulation in different cell types and species. Despite the known motifs/domains of Me31B, the in vivo functions of the motifs remain unclear. Here, we used the Drosophila germline as a model and used CRISPR to mutate the key Me31B motifs/domains: helicase domain, N-terminal domain, C-terminal domain and FDF-binding motif....
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