Article
Inter-laboratory evaluation of SNP-based forensic identification by massively parallel sequencing using the Ion PGM™.
Forensic science international. Genetics - 1 Jul 2015
Eduardoff M, Santos C, de la Puente M, Gross T E, Fondevila M, Strobl C, Sobrino B, Ballard D, Schneider P M, Carracedo Á, Lareu M V, Parson W, Phillips C
Abstract excerpt
Next generation sequencing (NGS) offers the opportunity to analyse forensic DNA samples and obtain massively parallel coverage of targeted short sequences with the variants they carry. We evaluated the levels of sequence coverage, genotyping precision, sensitivity and mixed DNA patterns of a prototype version of the first commercial forensic NGS kit: the HID-Ion AmpliSeq™ Identity Panel with 169-markers designed...
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