Article
Development of a one-pot RT-RAA/CRISPR-Cas13a assay for rapid genotyping of Nipah virus in pigs.
Diagnostic microbiology and infectious disease - 1 Jun 2026
Zhang Hui, Cui Chaojie, Wang Xiaohua, Liu Shuang, Wang Xiaozhen, Wang Yingli, Ge Shengqiang, Cai Yumei, Bao Jingyue, Wang Zhiliang
Abstract excerpt
INTRODUCTION: Nipah virus (NiV) is a highly pathogenic zoonotic virus transmitted from bats to humans through pigs as a key intermediate host. Given the existence of two distinct NiV genotypes, which differ in clinical manifestations and transmission patterns in both humans and pigs, rapid and sensitive method for detection and genotyping is crucial for effective disease control. Isothermal amplification combined...
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