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LT-RPA: An Isothermal DNA Amplification Approach for Improved Microsatellite Genotyping and Microsatellite Instability Detection.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Jeanjean Sophie I, Renault Victor, Daunay Antoine, Shen Yimin, Hardy Lise M, Deleuze Jean-François, How-Kit Alexandre

Abstract excerpt

Microsatellites are short tandem repeats of one to six nucleotides that are highly polymorphic and extensively used as genetic markers in numerous biomedical applications, including the detection of microsatellite instability (MSI) in cancer. The standard analytical method for microsatellite analysis relies on PCR amplification followed by capillary electrophoresis or, more recently, next-generation sequencing...

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PMID: 37041442
DOI: 10.1007/978-1-0716-2950-5_7

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