Article
Derived Polymorphic Amplified Cleaved Sequence (dPACS) Assay.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Kaundun Shiv Shankhar, Hutchings Sarah-Jane, Downes Joe, Baker Ken
Abstract excerpt
The derived polymorphic amplified cleaved sequence (dPACS) assay is a simple polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP)-based procedure for detecting known single-nucleotide polymorphisms (SNPs) and deletion-insertion polymorphisms (DIPs). It is relatively straightforward to carry out using basic and commonly available molecular biology kits. The method differs from other...
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