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Article

A modified high-resolution melting-based assay (HRM) to identify the SARS-CoV-2 N501Y variant.

Journal of virological methods - 1 Apr 2023

Aoki Akira, Adachi Hirokazu, Mori Yoko, Ito Miyabi, Sato Katsuhiko, Kinoshita Masayoshi, Kuriki Masahiro, Okuda Kenji, Sakakibara Toru, Okamoto Yoshinori, Jinno Hideto

Abstract excerpt

High-resolution melting (HRM) analysis is a PCR-based method that can be used as a screening assay to identify SARS-CoV-2 variants. However, conventional HRM assays hardly detect slight melting temperature differences at the A-T to T-A transversion. As the N501Y substitution results from A-T to T-A transversion in A23063, few or no studies have shown that a conventional HRM assay can identify N501Y variants. This...

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Identifiers

PMID: 36681163
DOI: 10.1016/j.jviromet.2023.114678
PMCID: PMC9847321

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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