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Article

Rapid and qualitative identification of SARS-CoV-2 mutations associated with variants of concern using a multiplex RT-PCR assay coupled with melting analysis.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases - 1 Sept 2022

Sberna Giuseppe, Fabeni Lavinia, Berno Giulia, Carletti Fabrizio, Specchiarello Eliana, Colavita Francesca, Meschi Silvia, Matusali Giulia, Garbuglia Anna Rosa, Bordi Licia, Lalle Eleonora

Abstract excerpt

OBJECTIVES: Considering the spread of new genetic variants and their impact on public health, it is important to have assays that are able to rapidly detect SARS-CoV-2 variants. METHODS: We retrospectively examined 118 positive nasopharyngeal swabs, first characterized by the Sanger sequencing, using the Simplexa® SARS-CoV-2 Variants Direct assay, with the aim of evaluating the performance of the assay to detect...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 35760381
DOI: 10.1016/j.ijid.2022.06.032
PMCID: PMC9233866

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