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Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens

16 Nov 2022

Abstract excerpt

Memory B cells (MBCs), part of the immune response elicited by infection or vaccination, can persist in lymphoid organs and peripheral blood and are capable of rapid reactivation upon secondary antigen exposure. Here, we describe a flow cytometric assay to identify antigen-specific MBCs from peripheral blood mononuclear cells and characterize their isotypes and activation status. We detail steps to use...

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PMID: 36595922
DOI: 10.1016/j.xpro.2022.101902

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