Article
Long-Term Culture of Individual Caenorhabditis elegans on Solid Media for Longitudinal Fluorescence Monitoring and Aversive Interventions.
Journal of visualized experiments : JoVE - 2 Dec 2022
Espejo Luis, Hull Bradford, Chang Leah M, DeNicola Destiny, Freitas Samuel, Silbar Vanessa, Haskins Anne, Turner Emily A, Sutphin George L
Abstract excerpt
Caenorhabditis elegans are widely used to study aging biology. The standard practice in C. elegans aging studies is to culture groups of worms on solid nematode growth media (NGM), allowing the efficient collection of population-level data for survival and other physiological phenotypes, and periodic sampling of subpopulations for fluorescent biomarker quantification. Limitations to this approach are the...
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