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Article

A viral metagenomic protocol for nanopore sequencing of group A rotavirus.

Journal of virological methods - 1 Feb 2023

Yandle Zoe, Gonzalez Gabriel, Carr Michael, Matthijnssens Jelle, De Gascun Cillian

Abstract excerpt

AIM: Development of an unbiased methodology using Oxford Nanopore Technology (ONT) sequencing to obtain whole-genome sequences (WGS) of Rotavirus A (RVA) from clinical samples. METHODS: 157 RVA qRT-PCR positive faecal samples were enriched by virus-like particle (VLP) purification and host nuclease digestion to enhance the detection of viral nucleic acids and cDNA generated as per the NetoVIR protocol. ONT...

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Identifiers

PMID: 36494024
DOI: 10.1016/j.jviromet.2022.114664

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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