Article
Population admixtures in medaka inferred by multiple arbitrary amplicon sequencing.
Scientific reports - 21 Nov 2022
Fujimoto Shingo, Yaguchi Hajime, Myosho Taijun, Aoyama Hiroaki, Sato Yukuto, Kimura Ryosuke
Abstract excerpt
Cost-effective genotyping can be achieved by sequencing PCR amplicons. Short 3-10 base primers can arbitrarily amplify thousands of loci using only a few primers. To improve the sequencing efficiency of the multiple arbitrary amplicon sequencing (MAAS) approach, we designed new primers and examined their efficiency in sequencing and genotyping. To demonstrate the effectiveness of our method, we applied it to...
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