Article
The translocated virulence protein VirD5 causes DNA damage and mutation during Agrobacterium-mediated transformation of yeast.
Science advances - 18 Nov 2022
Zhang Xiaorong, Hooykaas Marjolein J G, van Heusden G Paul, Hooykaas Paul J J
Abstract excerpt
The soil bacterium Agrobacterium tumefaciens is a preferred gene vector not only for plants but also for fungi. Agrobacterium delivers a small set of virulence proteins into host cells concomitantly with transferred DNA (T-DNA) to support the transformation process. Here, we find that expression of one of these proteins, called VirD5, in yeast host cells causes replication stress and DNA damage. This can result...
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