Article
VirD4-independent transformation by CloDF13 evidences an unknown factor required for the genetic colonization of plants via Agrobacterium.
Molecular microbiology - 1 Feb 2003
Escudero Jesús, Den Dulk-Ras Amke, Regensburg-Tuïnk Tonny J G, Hooykaas Paul J J
Abstract excerpt
Agrobacterium uses a mechanism similar to conjugation for trans-kingdom transfer of its oncogenic T-DNA. A defined VirB/VirD4 Type IV secretion system is responsible for such a genetic transfer. In addition, certain virulence proteins as VirE2 can be mobilized into host cells by the same apparatus. VirE2 is essential to achieve plant but not yeast transformation. We found that the limited host range plasmid...
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