Article
Quantification of hepatitis E virus in raw pork livers using droplet digital RT-PCR.
Food microbiology - 1 Feb 2023
Wang Kairui, Liu Libing, Wang Jinfeng, Sun Xiaoxia, Han Qingan, Zhou Cang, Xu Xiangdong, Wang Jianchang
Abstract excerpt
Hepatitis E virus (HEV) is the causative agent of hepatitis E. Some of the rise in hepatitis E infection in China may be linked to undercooked pork. In this study, we established a reverse transcription droplet digital PCR (RT-ddPCR) method to detect HEV in raw pork livers. The detection limit of the assay for HEV RNA was as low as 1.81 copies/μL. The suggested approach was validated on 14 samples, demonstrating...
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