Article
A suite of PCR-LwCas13a assays for detection and genotyping of Treponema pallidum in clinical samples.
Nature communications - 9 Aug 2022
Chen Wentao, Luo Hao, Zeng Lihong, Pan Yuying, Parr Jonathan B, Jiang Yinbo, Cunningham Clark H, Hawley Kelly L, Radolf Justin D, Ke Wujian, Ou Jiangli, Yang Jianjiang, Yang Bin, Zheng Heping
Abstract excerpt
The performance of commonly used assays for diagnosis of syphilis varies considerably depending on stage of infection and sample type. In response to the need for improved syphilis diagnostics, we develop assays that pair PCR pre-amplification of the tpp47 gene of Treponema pallidum subsp. pallidum with CRISPR-LwCas13a. The PCR-LwCas13a assay achieves an order of magnitude better analytical sensitivity than...
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