Article
Optimizing virus-induced gene silencing efficiency with Cymbidium mosaic virus in Phalaenopsis flower.
Plant science : an international journal of experimental plant biology - 1 Mar 2013
Hsieh Ming-Hsien, Lu Hsiang-Chia, Pan Zhao-Jun, Yeh Hsin-Hung, Wang Shyh-Shyan, Chen Wen-Huei, Chen Hong-Hwa
Abstract excerpt
Virus-induced gene silencing (VIGS) is a good way to study floral gene functions of orchids, especially those with a long life cycle. To explore the applicability and improve viral silencing efficiency for application of Cymbidium mosaic virus (CymMV)-induced gene silencing, we examined several variables, including the optimal length of the DNA fragment, the effect of developmental maturation status of...
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