Article
An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA.
Memorias do Instituto Oswaldo Cruz - 1 Feb 2016
Santos Ana Paula de Torres, Levi José Eduardo, Lemos Marcilio Figueiredo, Calux Samira Julien, Oba Isabel Takano, Moreira Regina Célia
Abstract excerpt
This study aimed to standardise an in-house real-time polymerase chain reaction (rtPCR) to allow quantification of hepatitis B virus (HBV) DNA in serum or plasma samples, and to compare this method with two commercial assays, the Cobas Amplicor HBV monitor and the Cobas AmpliPrep/Cobas TaqMan HBV test. Samples from 397 patients from the state of São Paulo were analysed by all three methods. Fifty-two samples were...
Topics
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Child
- Child, Preschool
- DNA Primers
- DNA, Viral
- Evaluation Studies as Topic
- Female