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Article

Hypermutation of specific genomic loci of Pseudomonas putida for continuous evolution of target genes.

Microbial biotechnology - 1 Sept 2022

Velázquez Elena, Álvarez Beatriz, Fernández Luis Ángel, de Lorenzo Víctor

Abstract excerpt

The ability of T7 RNA polymerase (RNAPT7 ) fusions to cytosine deaminases (CdA) for entering C➔T changes in any DNA segment downstream of a T7 promoter was exploited for hyperdiversification of defined genomic portions of Pseudomonas putida KT2440. To this end, test strains were constructed in which the chromosomally encoded pyrF gene (the prokaryotic homologue of yeast URA3) was flanked by T7 transcription...

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Identifiers

PMID: 35695013
DOI: 10.1111/1751-7915.14098
PMCID: PMC9437889

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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