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Article

Protocol to induce and assess cGAS-STING pathway activation in vitro

14 May 2022

Abstract excerpt

The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway plays a pivotal role in several cellular processes including pathogen recognition and inflammatory responses. We describe a protocol to activate the cGAS-STING pathway in murine cells using nucleic acids transfection. We describe how to prepare the nucleic acid probes and validate activation of the pathway by western blot and gene...

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PMID: 35600929
DOI: 10.1016/j.xpro.2022.101384

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