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Advancing the Rose Rosette Virus Minireplicon and Encapsidation System by Incorporating GFP, Mutations, and the CMV 2b Silencing Suppressor.

Viruses - 17 Apr 2022

Urrutia Cesar D, Romay Gustavo, Shaw Brian D, Verchot Jeanmarie

Abstract excerpt

Plant infecting emaraviruses have segmented negative strand RNA genomes and little is known about their infection cycles due to the lack of molecular tools for reverse genetic studies. Therefore, we innovated a rose rosette virus (RRV) minireplicon containing the green fluorescent protein (GFP) gene to study the molecular requirements for virus replication and encapsidation. Sequence comparisons among RRV...

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PMID: 35458566
DOI: 10.3390/v14040836
PMCID: PMC9031449

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