Novel method utilizing bisulfite conversion with dual amplification-refractory mutation system polymerase chain reaction to detect circulating pancreatic β-cell cfDNA.

doi:10.1111/jdi.13806

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Novel method utilizing bisulfite conversion with dual amplification-refractory mutation system polymerase chain reaction to detect circulating pancreatic β-cell cfDNA.

Journal of diabetes investigation - 1 Jul 2022

Okada Asami, Yamada-Yamashita Misuzu, Tominaga Yukari, Jo Kyoka, Mori Hiroyasu, Suzuki Reiko, Ishizu Masashi, Tamaki Motoyuki, Akehi Yuko, Takashi Yuichi, Koga Daisuke, Shimokita Eisuke, Tanihara Fuminori, Kurahashi Kiyoe, Yoshida Sumiko, Mitsui Yukari, Masuda Shiho, Endo Itsuro, Aihara Ken-Ichi, Kagami Shoji, Abe Masahiro, Ferreri Kevin, Fujitani Yoshio, Matsuhisa Munehide, Kuroda Akio

Abstract excerpt

AIMS/INTRODUCTION: Several research groups have reported methods for quantifying pancreatic beta cell (β-cell) injury by measuring β-cell-specific CpG unmethylation of the insulin gene in circulation using digital droplet PCR or next-generation sequencing. However, these methods have certain disadvantages, such as the need to consider the background signal owing to the small number of target CpG sites and the...

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PMID: 35396829
DOI: 10.1111/jdi.13806
PMCID: PMC9248415

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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