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Batch Effects during Human Bone Marrow Stromal Cell Propagation Prevail Donor Variation and Culture Duration: Impact on Genotype, Phenotype and Function.

Cells - 10 Mar 2022

Brachtl Gabriele, Poupardin Rodolphe, Hochmann Sarah, Raninger Anna, Jürchott Karsten, Streitz Mathias, Schlickeiser Stephan, Oeller Michaela, Wolf Martin, Schallmoser Katharina, Volk Hans-Dieter, Geissler Sven, Strunk Dirk

Abstract excerpt

Donor variation is a prominent critical issue limiting the applicability of cell-based therapies. We hypothesized that batch effects during propagation of bone marrow stromal cells (BMSCs) in human platelet lysate (hPL), replacing fetal bovine serum (FBS), can affect phenotypic and functional variability. We therefore investigated the impact of donor variation, hPL- vs. FBS-driven propagation and exhaustive...

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Identifiers

PMID: 35326396
DOI: 10.3390/cells11060946
PMCID: PMC8946746

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