Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Tracking SARS-CoV-2 variants by entire S-gene analysis using long-range RT-PCR and Sanger sequencing.

Clinica chimica acta; international journal of clinical chemistry - 1 May 2022

Matsubara Mirai, Imaizumi Yuri, Fujikawa Tatsuki, Ishige Takayuki, Nishimura Motoi, Miyabe Akiko, Murata Shota, Kawasaki Kenji, Taniguchi Toshibumi, Igari Hidetoshi, Matsushita Kazuyuki

Abstract excerpt

INTRODUCTION: Genomic surveillance of the SARS-CoV-2 virus is important to assess transmissibility, disease severity, and vaccine effectiveness. The SARS-CoV-2 genome consists of approximately 30 kb single-stranded RNA that is too large to analyze the whole genome by Sanger sequencing. Thus, in this study, we performed Sanger sequencing following long-range RT-PCR of the entire SARS-CoV-2 S-gene and analyzed the...

Topics

Join the communities discussing this publication.

COVID-19
Mutation
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Open in PubMed Open full text

Identifiers

PMID: 35304093
DOI: 10.1016/j.cca.2022.03.014
PMCID: PMC8923710

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Tracking SARS-CoV-2 variants by entire S-gene analysis using long-range RT-PCR and Sanger sequencing.PMID 35304093Read ↗

Molecular biology of SARS-CoV-2 and techniques of diagnosis and surveillance.Semantic proximity Read ↗

Monitoring the spread of the SARS-CoV-2 (Coronaviridae: Coronavirinae: Betacoronavirus; Sarbecovirus) variants in the Moscow region using targeted high-throughput sequencingSemantic proximity Read ↗

Evidence-Based Evaluation of PCR Diagnostics for SARS-Cov-2 and The Omicron Variants by Sanger SequencingSemantic proximity Read ↗

Implementation of an In-House Platform for Rapid Screening of SARS-CoV-2 Genome Variations.Semantic proximity Read ↗

PCR-Sequencing Approaches to Assess Informative Mutations in SARS-Cov-2 Spike (S) and ORF7, ORF8 and N Genes Characterizing Variants of Concern and Variants of InterestSemantic proximity Read ↗

Identification of SARS-CoV-2 Variants Using Viral Sequencing for the Centers for Disease Control and Prevention Genomic U.S. Surveillance Program.Semantic proximity Read ↗

RT-PCR genotyping assays to identify SARS-CoV-2 variants in England in 2021: a design and retrospective evaluation study.Semantic proximity Read ↗

Identification of SARS-CoV-2 variants using viral sequencing for the Centers for Disease Control and Prevention genomic surveillance programSemantic proximity Read ↗

Efficient SARS-CoV-2 Surveillance during the Pandemic-Endemic Transition Using PCR-Based Genotyping Assays.Semantic proximity Read ↗

Evaluation of a Rapid and Accessible RT-qPCR Approach for SARS-CoV-2 Variant of Concern IdentificationSemantic proximity Read ↗

Quasispecies of SARS-CoV-2 revealed by single nucleotide polymorphisms (SNPs) analysis.Semantic proximity Read ↗

A program for real-time surveillance of SARS-CoV-2 geneticsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.