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Analysis and comparison of the STR genotypes called with HipSTR, STRait Razor and toaSTR by using next generation sequencing data in a Brazilian population sample.

Forensic science international. Genetics - 1 May 2022

Valle-Silva Guilherme, Frontanilla Tamara Soledad, Ayala Jesús, Donadi Eduardo Antonio, Simões Aguinaldo Luiz, Castelli Erick C, Mendes-Junior Celso Teixeira

Abstract excerpt

Short tandem repeats (STRs) are particularly difficult to genotype with rapid evolving next-generation sequencing (NGS) technology. Long amplicons containing repetitive sequences result in alignment and genotyping errors. Stutters arising from polymerase slippage often result in reads with additional or missing repeat copies. Many tools are available for analysis of STR markers from NGS data. This study has...

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PMID: 35144075
DOI: 10.1016/j.fsigen.2022.102676

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