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Article

Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA.

Emerging infectious diseases - 1 Apr 2022

Heguy Adriana, Dimartino Dacia, Marier Christian, Zappile Paul, Guzman Emily, Duerr Ralf, Wang Guiqing, Plitnick Jonathan, Russell Alexis, Lamson Daryl M, St George Kirsten

Abstract excerpt

Of 379 severe acute respiratory syndrome coronavirus 2 samples collected in New York, USA, we detected 86 Omicron variant sequences containing Delta variant mutation P681R. Probable explanations were co-infection with 2 viruses or contamination/amplification artifact. Repeated library preparation with fewer cycles showed the P681R calls were artifactual. Unusual mutations should be interpreted with caution.

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Identifiers

PMID: 35130474
DOI: 10.3201/eid2804.220146
PMCID: PMC8962901

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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