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AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses

20 Jan 2022

Abstract excerpt

assembly of recombinant vectors from plasmid inserts that span the adenovirus genome. Two advantages of AdenoBuilder are the ease of modifying the genome and the ability to produce multicomponent vectors in a single step, facilitating parallel approaches to vector optimization. This protocol describes how to introduce transgenes in place of the endogenous Human Adenovirus serotype 5 (HAd5) E1 and/or E3 genes and...

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PMID: 35098167
DOI: 10.1016/j.xpro.2022.101123

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