Article
Amino acid substitutions at the HIV-1 transframe region significantly impair virus infectivity.
PloS one - 1 Jan 2022
Yu Fu-Hsien, Huang Kuo-Jung, Wang Chin-Tien
Abstract excerpt
A transframe region within HIV-1 Gag-Pol (referred to as p6* or p6pol), directly linked to the protease (PR) N-terminus, plays a pivotal role in modulating PR activation. To identify specific p6* residues involved in PR activation, we created a series of p6* mutants by making substitutions for conserved p6* residues. Our results indicate that some p6* mutants were defective in terms of virus infectivity, despite...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Cell Line
- Cell Line, Tumor
- Fusion Proteins, gag-pol
- HEK293 Cells
- HIV Protease
- HIV Seropositivity
- HIV-1
- HeLa Cells
- Humans
- Mutation
- Virion
- Virus Replication