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Single-cell DNA and RNA sequencing of circulating tumor cells

24 Nov 2021

Abstract excerpt

Single-cell sequencing of circulating tumor cells can precisely represent tumor heterogeneity and provide useful information for cancer treatment and research. After spiking TGW neuroblastoma cells into blood derived from healthy volunteer, the cells were isolated by fluorescence-activated cell sorting. DNA and mRNA were amplified by four different whole-genome amplifications (WGA) and three whole-transcriptome...

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PMID: 34819539
DOI: 10.1038/s41598-021-02165-7

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