Article
A Rapid Drug Resistance Genotyping Workflow for Mycobacterium tuberculosis, Using Targeted Isothermal Amplification and Nanopore Sequencing.
Microbiology spectrum - 22 Dec 2021
Gliddon Harriet D, Frampton Dan, Munsamy Vanisha, Heaney Jude, Pataillot-Meakin Thomas, Nastouli Eleni, Pym Alexander S, Steyn Adrie J C, Pillay Deenan, McKendry Rachel A
Abstract excerpt
Phenotypic drug susceptibility testing (DST) for tuberculosis (TB) requires weeks to yield results. Although molecular tests rapidly detect drug resistance-associated mutations (DRMs), they are not scalable to cover the full genome and the many DRMs that can predict resistance. Whole-genome sequencing (WGS) methods are scalable, but if conducted directly on sputum, typically require a target enrichment step, such...
Topics
- Antitubercular Agents
- Drug Resistance, Bacterial
- Genotype
- Humans
- Isoniazid
- Mycobacterium tuberculosis
- Nanopore Sequencing
- Polymerase Chain Reaction
- Rifampin
- Sensitivity and Specificity
- Sputum
- Tuberculosis, Multidrug-Resistant