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High-throughput nanopore targeted sequencing for efficient drug resistance assay of Mycobacterium tuberculosis

22 May 2024

Abstract excerpt

Drug-resistant tuberculosis (TB), especially multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB), is one of the urgent clinical problems and public health challenges. Culture-based phenotypic drug susceptibility testing (pDST) is time-consuming, and PCR-based assays are limited to hotspot mutations. In this study, we developed and validated a convenient and efficient...

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PMID: 38841074
DOI: 10.3389/fmicb.2024.1331656

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