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Article

Preparation of multiplexed control materials for cancer mutation analysis by genome editing in GM12878 cells.

Journal of clinical laboratory analysis - 1 Jan 2022

Lin Guigao, Zhang Kuo, Han Yanxi, Peng Rongxue, Li Jinming

Abstract excerpt

BACKGROUND: Quality control materials are necessary for assay development, test validation, and proficiency testing in cancer mutation analysis. Most of the existing controls for somatic mutations only harbor a single variant and are derived from unstable cell lines. This study aimed to establish a method to create stable multianalyte controls in a defined background by genome editing in GM12878 cells, which also...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34811797
DOI: 10.1002/jcla.24139
PMCID: PMC8761438

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